ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
German cockroach (Blattella germanica), the dominant species of cockroaches in China, has an important role in research due to its significance and harmfulness in medicine and economics and being a preponderant research model for many scientific problems. Functional gene research has been the basis for biological research in the post-genome era. RNA interference (RNAi) is a post-transcriptional gene (mRNA) silencing where endogenous or exogenous double-strand RNA specifically and efficiently suppresses the expression of a functional gene at cellular and gross levels. In view of the research value of cockroaches and the technical advantage of RNAi, this article briefly reviews the molecular mechanism of RNAi, with an emphasis on the latest research on the functional genes of B. germanica.
Wide application of insecticides has resulted in severe resistance in the German cockroach (Blattella germanica). This study reviews the discovery and molecular and evolutional mechanisms of the knockdown resistance (kdr), also known as the sodium channel resistance, with emphasis on the molecular structure, functional research Methods of kdr mutations and up-to- date representative results. In addition, the latest findings are summarized to inform the prevention, control and resistance monitoring of the cockroaches.
Objective To study the activity of Glutathione S-transferase (GSTs) of German cockroaches (Blattella germanica) at different developmental stages (from nymph to adult) and different field strains in Beijing. Methods The specific activity of GSTs and resistance levels of B. germanica were determined by spectrophotometry and contact biossay. Results The specific activity of GSTs increased gradually during the nymph period and reached the highest by week 4, which then decreased to the lowest value during eclosion. The specific activity of GSTs in susceptible strains of B. germanica was 2.70 nmol/(min·mg) with Km of 2.26 mmol/L and Vmax of 0.33 nmol/(min·mg); the specific activity of GSTs in nine field strains ranged from 2.76-8.69 nmol/(min·mg) with Km of 0.65-2.37 mmol/L and Vmax of 0.33-0.54 nmol/(min·mg) Vmax. Compared with susceptible strains, field strains had higher specific activity and Vmax yet lower Km values. Field strains also demonstrated different degrees of resistance to two commonly used pesticides (cypermethrin 1.50-8.37 fold; propoxur 1.04-3.34 fold). Conclusion GST activity varied in the developmental stages from nymph to adult, which may be involved in the development of insecticide resistance in the field strains of B. germanica.
Objective To examine vector prevention and control practices for large-scale events in China by describing vector monitoring and control as a part of pest control operations (PCO) in the 2008 Beijing Olypmic Games. Methods Candidate PCO companies were first screened, and eligible bid winners were then trained and tested, during which the services provided were supervised and inspected by the government designated agency. Results The PCO companies achieved satisfying vector control rates during the 2008 Olympic Games. Conclusion A government-funded pest control model for large-scale events has been established, which utilizes well-trained PCO companies under the management, coordination and supervision of health care administrators knowledgable of technical protocols formulated by the disease prevention and control system.
Objective To identify the body and subcellular distribution of acetylcholinesterase (AChE) activities in the Blattella germanica. Methods The AChE activities in different body parts and subcells were determined. Results The AChE activities in the head, chest and abdomin of male adults were 56.9%, 19.5% and 23.6% of the total activity, respectively. The difference in activity between the three body parts was statistically significant (F=127.96,P<0.05), where the AChE activity in the head was significantly higher than those in the chest and abdomen (P<0.001). The subcellular organelles in descending order of the total and specific AChE activity were: mitochondria>microsomes>cytoplasmic solution>nuclei and cell debris. Conclusion AChE activities were distributed in the head, chest and abdomen of B. germanica, with the highest in the head accounting for more than 50% of the overall activity. Subcellular distribution showed the highest AChE activity distribution in the mitochondria, accounting for almost 50% of the total activity.
【Abstract】 Objective To analyse effect factors of trap method and bait method, and to discuss how to improve the accuracy of surveillance methods. Methods Two methods combined with powder trace method were used in this study and its effect factors were analyzed. Results The invalid areas surveyed by bait method accounted for 53%, suggesting that the inducement of sweet potato was very weak. Successful surveillance area accounted for 8% and 67% by trap method and powder?trace method, respectively, showing that the reason leading to the insensitivity of rodent to trap method was its strong repellence to new substance. Conclusion There are many factors to affect the surveillance of rodent such as the repellence of rodent to new substance, the lure of baits, the abundance of food, the sensitivity of surveillance facility and the layout of surveillance sites. It is good to improve the accuracy of surveillance by single factor analysis.
【Abstract】 Objective To screen resistance?related gene from the susceptible strains and field resistant strains of Blattella germanica by comparing their gene expression products. Methods Based on the known B.germanica sequence information released by NCBI database, the oligonucleotide probes were designed and synthesized. The oligonucleotide microarray was employed to analyze the expression profile of susceptible and resistant strains and differential expression genes associated with resistance were screened out. The results were confirmed by real time RT?PCR. Results Five differential expression genes were screened out, including three of up?regulated genes (fold change≥2) such as CYP6K1, alpha?amylase mRNA and aspartic protease precursor and two of down?regulated genes (fold change≤0.5) like allergen Bla g 6.0101 mRNA and allergen Bla g 8 mRNA. Conclusion It is possible to screen out differential expression gene by self?made gene chip, and CYP6K1 is perhaps closely related to resistance.
【Abstract】 Objective To know about the mosquito density in Beijing city and Olympic venues by questionnaire. Methods Seven districts and seven Olympic venues were chosen to implement questionnaire survey by stratified random sampling. Results According to survey in the indoor environment, 92.31% of the respondents thought there were only a few mosquitoes in Beijing city, and 96.02% of the respondents thought there were few mosquitoes in Olympic venues. For the outdoor environment, there were 77.81% of the respondents who considered few mosquitoes in Beijing and 97.26% of the respondents considered that few mosquitoes was in the Olympic venues. About 69.85% of the respondents thought there were fewer mosquitoes in 2008 than in 2007 in Beijing. There were 80.60% of the respondents considered Olympic venues were good. Conclusion The mosquito density in both the indoor and outdoor environment in Olympic venues was lower than that in Beijing city, and the mosquito density in 2008 was lower than that in 2007.
【Abstract】 Objective To summarize the successful experience and disadvantage for Beijing Olympic Game by analyzing the integrated vector management (IVM) strategy in green homeland media village. Methods The integrated management measure was used in this study in which the principles like “devoting main efforts into source reduction, putting chemical control first and combining physical control with environment management”. Results Random investigation showed that above 80% people selected no vectors or seldom vectors. The density of mosquito and fly declined quickly, and the average densities of them were 18.5/lamp·2 h and 29.4/cage·d, which was little higher than that in the other nuclear areas. Rodent and cockroach density was 0. Conclusion It is essential to define organization, carry out the responsibilities and eliminate the breeding site inside and outside of green homeland media village.
【Abstract】 Objective To know about West Nile virus (WNV) in mosquitoes during 2008 Beijing Olympic Games, and prevent and control West Nile fever. Methods Mosquitoes were collected by CO2 lamp trapping method. Two methods, ADAPCO’s Ramp System and MAS’s VecTest Kits, were used to detect WNV in mosquitoes. Results Compared with 2006 and 2007, the density of main vectors decreased significantly in 2008. Culex pipiens pallens was the dominant specie of mosquito, which constituted ratio reached 96.94% in Beijing Olympic venues. There were 22 275 mosquitoes captured in Beijing Olympic venues that all were negative by detection. Conclusion Mosquitoes were controlled effectively in 2008 Olympic Games. No WNV?positive mosquitoes were detected.
【Abstract】 Objective To establish the monitoring system and network of the vector and know the flies density dynamics and its population distribution in the early reconstruction after earthquake in Shifang city. Methods The fly density and its population composition were monitored in tents, removable house, trash can, toilet and hospitals by cage trapping method in 5 towns in Shifang city. Results The flies caught mainly belonged to 3 species,6 genera. Of which, Chrysomya megacephala was the dominant specie, accounting for 56.0% of the total. In monitored 5 township (town), the density of fly was higher in Luoshui town and Yinghua town, with the density of 358.0 individuals/cage and 91.7 individuals/cage , and the density of flies in Mazu town was the lowest with the density of 13.0 individuals/cage. Conclusion This investigation primarily knew the flies density and its population composition after earthquake in Shifang and filled a gaps in this area, which could provided the basis for the prevention and control of flies.
【Abstract】 Objective To predict the risk indicators of the harm caused by vectors and the occurrence of related diseases during the Olympic Games by risk recognition and assessment methods. To take response measure timely and reduce effectively the harm caused by vectors in order to prevent the occurrence and prevalence of vector?borne diseases. Methods To grade the risk by analyzing the occurrence probability and consequence of risk event comprehensively. Firstly, educe the list and confirm the classification of the risk by literature consultation, empirical analysis, brain storm and expert advice. Secondly, judge the risk level by risk assessment index matrix method and provide the standard to the risk classification. Results The vector?borne diseases and nuisance caused by vectors could be potential during the Olympic Games. For the probability of the public health event caused by vectors, it certainly happens for the biting and infestation of vectors, and probably occurs for environment disruption. It is rare for the occurrence of cables bitten by rodent and power apparatus disruption. The levels of the public health event damaged by vector are as follows: it is moderate harm for the cable bitten and electronic facility disruption, and it is slight harm for the vector biting and infestation or environment disruption. Conclusion The goal that there is no serious harmful events caused by vectors during Beijing Olympic Games would be certainly realized by scientific recognition and assessment of the risk, comprehensive surveillance of vectors, integrated control, scientific using of pesticide, the organization of emergency response team and the generalization of advanced technology.
【Abstract】 Objective To know about the density and seasonal fluctuation of main vectors at National Stadium by the surveillance, develop and implement the special strategy and control, in order to avoid the harmfulness caused by vectors during the opening and closing ceremony of Olympic Games. Methods Night trapping method was used for the surveillance of rats, CO2 trapping lamp method for the adult mosquitoes, lava-spoon method and check-container method for larva, baited cage trapping method for the flies and paste catching method for cockroaches. The integrated method was used for the vector prevention and control such as rats, mosquitoes, flies and cockroaches. The control effect was evaluated by density surveillance and questionnaire. Results There were 84 spoons sampled from large-scale water, and the positive spoon was 0. A total of 46 containers were checked at the stadium, and no positive container was found. There was no response on the infestation of vectors at the opening and closing ceremony of the Olympic Games by questionnaires to 220 people, and nobody was bitten or annoyed by vectors. Conclusion The strategy and method of vector control especially at the opening and closing ceremony of Olympic Games were successful and effective, which not only provided the safeguard for the opening and closing ceremony but also realized the aim without vector harm.
【Abstract】 Objective To know about the population distribution and seasonal fluctuation of main vectors by the successive monitoring from 2006 to 2008 in Beijing Olympic venues, and to provide the scientific basis for vector control. Methods The CO2 trapping lamp method was used for the surveillance of mosquitoes, baited cage trapping method for the flies, night trapping method for rats and paste catching method for cockroaches. Results Culex pipiens pallens was the dominant species of mosquito, accounting for 96.94% of the caught mosquitoes in Beijing Olympic venues. The dominant species of fly was Sarcophaginae, accounting for 46.13 % of the caught flies. Mus musculus Linnaeus was the dominant species of rats, accounting for 63.30 % of the caught rats. Blattella germanica was the dominant species of cockroaches, and all the captured cockroaches were German cockroaches. Compared with 2006 and 2007, the density of main vectors decreased significantly in 2008. During the period of the Olympic Games, the density of main vectors had been in the low level. The density of mosquito declined 88.57% and 89.26%, 69.88% and 81.63% for the fly density, 100% for the rat density and 99.98% for the cockroach density. Conclusion The density of main vectors decreased significantly during the period of Beijing Olympic Games. No serious harmful events caused by vectors occurred in Beijing 2008 Olympic Games. The goal about vector control had been realized completely.
【Abstract】 Objective To study the influence of main meteorological parameters on the mosquito density. Methods With the data of mosquito density from 2005 to 2007 provided by Beijing Center for Disease Control and Prevention and the meteorological data such as average temperature, average minimum temperature, average maximum temperature, rainfall, rainy days, sunshine time, relative humidity and average wind speed, a multiple stepwise regression would be made with statistical software SAS 9.0. Results The multiple regression co-linearity results showed there was strong linear relationship among average temperature,average minimum temperature and average minimum temperature. The multiple stepwise regression equation was y=0.533 97X2+0.078 14X7-2.673 29X8-2.232 56 (X2, X7 and X8 represented average minimum temperature, relative humidity and average wind speed, respectively). Conclusion The main meteorological parameters closely related to mosquito density were average minimum temperature, relative humidity and average wind speed. The order which affected mosquito density was in turn average minimum temperature, average wind speed and relative humidity